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KMID : 1140120070120010035
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Cancer Prevention Research
2007 Volume.12 No. 1 p.35 ~ p.40
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Suppressed Synthesis of Reactive Oxygen Species from HL60 Cell by Nicotine
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Lim Su-Jin
Kim Yong-Seok
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Abstract
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Reactive oxygen species (ROS) include superoxide anion radical (O2-.), singlet oxygen (1O2), hydrogen peroxide (H2O2), and the highly reactive hydroxyl radical (.OH) which are byproducts of the metabolism of oxygen. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-a cytochromeb-558-containing, plasma-membrane-bound enzyme complex-synthesizes superoxide anion which is quickly transformed into hydrogen peroxide and hydroxyl radical. ROS normally exist in all aerobic cells in balance with cellular antioxidants. Excess ROS or antioxidants depletion can evoke oxidative stress because of disruption of the balance. ROS can cause tissue damage by reacting with membrane lipids, DNA, and proteins. Oxidative stress is implicated in various diseases such as atherosclerosis, chronic inflammation, diabetes, ischemia-reperfusion injury, and malignant diseases. Nicotine, in tobacco smoking concentrations, is a powerful psychoactive drug. A wide variety of stimulant and depressant effects is observed in animals and humans that involves the central and peripheral nervous, cardiovascular, endocrine, gastrointestinal, and skeletal motor systems. In spite of these heterogenous effects, nicotine was confirmed to decrease superoxide anion from HL60 cell. Even when HL60 was treated with nicotine and other smoking components such as benzopyrene and cotinine together, the generation of superoxide
anion was completely suppressed by nicotine. This phenomenon was compared to the decreased superoxide anion from HL60 by toluene diisocyanates (TDI). TDI is supposed to remove superoxide anion from HL60 because nitric oxide induced by TDI can react with superoxide anion to form peroxynitrite anion (ONOO-). However, superoxide anion generation was initially and completely blocked by nicotine in comparison with the gradual decrease of superoxide anion by TDI. So, nicotine was regarded to be a particular suppressant of superoxide anion and could be used in conjunction with
antioxida nts in the prevention or therapy of diseases which have free radical reactions as an underlying mechanism of injury. (Cancer Prev Res 12, 35-40, 2007)
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KEYWORD
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Reactive oxygen species, Nicotine, HL60 cell, Antioxidants, Toluene diisocyanates
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